Plasmid

Part:BBa_K4127002:Design

Designed by: Xiongbing Yang   Group: iGEM22_CSU_CHINA   (2022-10-11)


E gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 477
    Illegal EcoRI site found at 657
    Illegal XbaI site found at 484
    Illegal PstI site found at 35
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 477
    Illegal EcoRI site found at 657
    Illegal PstI site found at 35
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 477
    Illegal EcoRI site found at 657
    Illegal BamHI site found at 53
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 477
    Illegal EcoRI site found at 657
    Illegal XbaI site found at 484
    Illegal PstI site found at 35
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 477
    Illegal EcoRI site found at 657
    Illegal XbaI site found at 484
    Illegal PstI site found at 35
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The plasmid can be expressed normally in Escherichia coli.


Source

The characteristic sequence of E gene, from the COVID-19 genome.

References